Nicorandil alleviates inflammation and oxidation in diabetic cardiomyopathy

Purpose: To examine the effect of nicorandil on high glucose-induced cardiomyocyte inflammation and oxidative stress.Methods: H9C2 cardiomyocytes were divided into control group, glucose group group. The survival rate was determined using CCK-8 method. contents reactive oxygen species (ROS) by flow cytometry. MDA LDH in cell supernatant kit. Western blot real-time PCR used to assess stress, apoptosis related factors each cardiomyocytes. expression levels IL-1β immunofluorescence. Tunnel staining determine level group.Results: expressions SOD1 SOD2 significantly decreased (p < 0.05). Also, increased Furthermore, IL-1β, TNF-α, caspase 3 Bax increased, while Bcl-2 inhibited. fluorescence also significantly. NF-κB Ikkα IκB-α inhibited stress apoptosis, as well pathway downstream factor Ikkα.Conclusion: Nicorandil ameliorates damage induced glucose, inhibiting pathway, thereby lowering apoptosis. Thus, findings provide new insight development agents for treatment diabetic cardiomyopathy.